Problem+Set+7

Here is the ProblemSet #7 file. The online due date is 07-15-2010. Please download it, answer the questions assigned to your LG (LG-WX, LG from Worcester; LG-MX, LG from Manchester), and upload your answer file back accordingly (below). If you have any problems to upload your file back, please send your file directly to me at jonathan.sheng@mcphs.edu and l will do it for you. Each question will be answered by two LGs independently.

Question A.(1) LG-M7:

Look at reaction 2:

Reaction is a hydrolysis

reference: []

LGW7: Problem Set #7 (Dr. Sheng; PSW380) Date of in class submission: 07/14/2010 Your Name (last, First): Ako-Nai, Stephen


 * (1). Amidase**



The reaction is hydrolysis.

Reference: []

Question A.(2) LG-M8: A. (2). NADPH-quinone oxidoreductase (DT-diaphorase) Answer: NADPH + Quinone <-> NADP + Semiquinone Reduction (Wikipedia)

LG-W8:

Question A. (3) LG-M9:

A. Please find a sample reaction (chemical equation) catalyzed by each of the following enzymes and indicate what type of reaction (oxidation, reduction, hydrolysis, etc.) it is. Feel free to use textbooks, internet search engines (Wikipedia, google, etc.) but please mention in your answers.

(3) Lactoperoxidase

Br- + H202 H20 + BrO- Oxidation (Wikipedia)

LG-W9: SCN- + H2O2 -> OSCN- Oxidation (Wikipedia) Lactoperoxidase -

Lactoperoxidase catalyzes the hydrogen peroxide (H2O2) oxidation of several acceptor molecules

acceptor + H2O2 → oxidized acceptor + H2O thiocyanate (SCN–) + hydrogen peroxide →hypothiocyanite(OSCN–) + water.

Question A (4) LG-W10:

Myeloperoxidase: Myeloperoxidase is an enzyme which catalyzes the oxidation of hydrogen peroxide with chloride ions to produce hypochlorite ion (OCl) (Wikipedia). H2O2 + Cl− -> HOCl (hypochlorous acid) + OH− HOCl -> H+ + OCl−

LG-W11: Myeloperoxidase (MPO), represented as either native enzyme, compound I and compound II, is an enzyme that can oxidize the reaction of hydrogen peroxide with halides to form hypohalous acids and plays an important role in neutrophil cells. In this example, MPO combines hydrogen peroxide with a chlorine ion to form hypochlorous acid. This reaction is an oxidation reaction. Reference: []

Question A.(5) LG-W12:



LG-W13:

(5). Catalase

H2O2 + RH2 > 2H2O + R

//A peroxidative reaction// provided by []

Example:

H2O2 + H2CO > 2 H2O + CO (Oxidation) (From Wikipedia)

Question A. (6) LG-W14: Amine Oxidase: Oxidation (from Wikipedia) RCH2NH2 + H2O + O2 -> RCHO + NH3 + H2O2

LG-W15:

Question A. (7) LG-W16:



LG-W17:

Qetion A. (8) LG-W18:

LG-W19: